Publications
- Musisi, E., Wamutu, S., Ssengooba, W., Kasiinga, S., Sessolo, A., Sanyu, I., Kaswabuli, S., Zawedde, J., Byanyima, P., Kia, P., Muwambi, W., Toskin, D. T., Kigozi, E., Walbaum, N., Dombay, E., Legrady, M. B., Ssemambo, K. D.-M., Joloba, M., Kuchaka, D., … Sabiiti, W. (2024). Accuracy of the tuberculosis molecular bacterial load assay to diagnose and monitor response to anti-tuberculosis therapy: a longitudinal comparative study with standard-of-care smear microscopy, Xpert MTB/RIF Ultra, and culture in Uganda. The Lancet Microbe, 5(4), e345-e354. https://doi.org/10.1016/S2666-5247(23)00367-1
- Danso, E.K., Asare, P., Osei-Wusu, S., Tetteh, P., Tetteh, A.Y., Boadu, A.A., Lamptey, I.N.K., Sylverken, A.A., Obiri-Danso, K., Afriyie Mensah, J., Adjei, A., Yeboah-Manu, D. (2024) Tuberculosis patients with diabetes co-morbidity experience reduced Mycobacterium tuberculosis complex clearance. Heliyon, Volume 10, Issue 15, e35670, ISSN 2405-8440. https://doi.org/10.1016/j.heliyon.2024.e35670
- Musisi, E., Mtafya, B., Wambi, W. S., Zawedde, J., Sessolo, A., Ssengooba, W., Walbaum, N., Ntinginya, N. E., Gillespie, S. H., & Sabiiti, W. (2024). Improved Diagnosis and Treatment Monitoring of Tuberculosis Using Stool and the Tuberculosis Bacterial Load Assay (TB-MBLA). Methods in Molecular Biology, 2833, 153-160. https://doi.org/10.1007/978-1-0716-3981-8_15
- Mtafya, B., Musisi, E., Qwaray, P., Sichone, E., Walbaum, N., Ntinginya, N. E., Gillespie, S. H., & Sabiiti, W. (2024). Quantifying Viable M. tuberculosis Safely Obviating the Need for High Containment Facilities. Methods in Molecular Biology, 2833, 145-152. https://doi.org/10.1007/978-1-0716-3981-8_14
- Nakiboneka, R., Walbaum, N., Musisi, E., Nevels, M., Nyirenda, T., Nliwasa, M., Msefula, C. L., Sloan, D., & Sabiiti, W. (2024). Specific human gene expression in response to infection is an effective marker for diagnosis of latent and active tuberculosis. Scientific Reports, 14, Article 26884. https://doi.org/10.1038/s41598-024-77164-5
- Gillespie, S. H., & Sabiiti, W. (2024). Tuberculosis molecular bacterial load assay in the management of tuberculosis. Current Opinion in Infectious Diseases. Advance online publication. https://doi.org/10.1097/QCO.0000000000001084
- Gillespie, S. H., DiNardo, A. R., Georghiou, S. B., Sabiiti, W., Kohli, M., Panzner, U., … & Heinrich, N. (2024). Developing biomarker assays to accelerate tuberculosis drug development: defining target product profiles. The Lancet Microbe. https://doi.org/10.1016/S2666-5247(24)00085-5
- Nakiboneka, R. F., & Sabiiti, W. (2024). The role and implications of RNAscope and mRNA in the diagnosis of tuberculosis. EBioMedicine, 105. https://doi.org/10.1016/j.ebiom.2024.105230
- Sessolo, A., Musisi, E., Kaswabuli, S., Zawedde, J., Byanyima, P., Sabiiti, W., Walimbwa, S., Ola, J., Sanyu, I., Lalitha, R., Kamya, M., Davis, L., Worodria, W., & Huang, L. (2023). Diagnostic accuracy of Xpert MTB/RIF Ultra and culture assays to detect Mycobacterium Tuberculosis using OMNIgene-sputum processed stool among adult TB presumptive patients in Uganda. PLoS One, 18(4), Article e0284041. https://doi.org/10.1371/journal.pone.0284041
- Musisi, E., Wyness, A. J., Eldirdiri, S., Dombay, E., Mtafya, B. A., Ntinginya, N. E., Heinrich, N., Kibiki, G. S., Hoelscher, M., Boeree, M., Aarnoutse, R., Gillespie, S. H., Sabiiti, W., & PanACEA Consortium (2023). Effect of seven anti-tuberculosis treatment regimens on sputum microbiome: a retrospective analysis of the HIGHRIF study 2 and PanACEA MAMS-TB clinical trials. The Lancet Microbe, 4(11), E913-E922. https://doi.org/10.1016/S2666-5247(23)00191-X
- Ntinginya, N. E., Bakuli, A., Mapamba, D., Sabiiti, W., Kibiki, G., Minja, L. T., Kuchaka, D., Reither, K., Phillips, P., Boeree, M. J., Gillespie, S. H., Hoelscher, M., Heinrich, N., & PanACEA Consortium (2023). Tuberculosis Molecular Bacterial Load Assay reveals early delayed bacterial killing in patients with relapse. Clinical Infectious Diseases, 76(3), e990-e994. Article ciac445. https://doi.org/10.1093/cid/ciac445
- Mtafya, B., Qwaray, P., John, J., Sichone, E., Shoo, A., Gillespie, S. H., Ntinginya, N. E., & Sabiiti, W. (2023). Corrigendum to “A practical approach to render tuberculosis samples safe for application of tuberculosis molecular bacterial load assay in clinical settings without a biosafety level 3 laboratory” [Tuberculosis 138 (2023) 102275 1-5] (Tuberculosis (2023) 138, (S1472979222001123), (10.1016/j.tube.2022.102275)). Tuberculosis, 142, Article 102321. https://doi.org/10.1016/j.tube.2023.102321
- Kontsevaya, I., Cabibbe, A. M., Cirillo, D. M., DiNardo, A. R., Frahm, N., Gillespie, S. H., Sabiiti, W. … & Goletti, D. (2023). Update on the diagnosis of tuberculosis. Clin Microbiol Infect. https://doi.org/10.1016/j.cmi.2023.07.014
- Ntinginya NE, Bakuli A, Mapamba D, Sabiiti W, Kibiki G, Minja L. T., … & Heinrich N; Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA) Consortium. (2023). Tuberculosis Molecular Bacterial Load Assay Reveals Early Delayed Bacterial Killing in Patients With Relapse. Clin Infect Dis. 76(3):e990-e994. https://doi.org/10.1093/cid/ciac445
- Musisi, E., Ssesolo, A., Sloan, D. J., Gillespie, S. H., & Sabiiti, W. (2022). Detection and quantification of viable Mycobacterium tuberculosis bacilli in saline-processed stool samples by Tuberculosis Molecular Bacterial Load Assay: a potential alternative for processing stool. Microbiology Spectrum, 10(3), Article 00274-22. https://doi.org/10.1128/spectrum.00274-22
- Dhasmana, D., Walbaum, N., Sabiiti, W., Gillespie, S., Sloan, D. (2022) P3 ECBS study: exacerbation of chronic bronchial sepsis – utility of a novel rapid molecular diagnostic test (MBLA) to detect and quantify viable bacteria. Thorax 77 (Suppl 1):A82.1-A82. DOI: 10.1136/thorax-2022-BTSabstracts.139
- Musisi E, Sessolo A, Kaswabuli S, Zawedde J, Byanyima P, Kasinga S, Sanyu I, Uwimaana E, Walimbwa S, Olore J, Ssengooba W, Sekaggya C, Joloba ML, Worodria W, Huang L,,Gillespie SH, Sloan DJ, Sabiiti W. (2022). High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of Tuberculosis. Microbiol Spectr10:e02100-21. https://doi.org/10.1128/spectrum.02100-21
- Mbelele, P. M., Sabiiti, W., Heysell, S. K., Sauli, E., Mpolya, E. A., Mfinanga, S., Gillespie, S. H., Addo, K. K., Kibiki, G., Sloan, D. J., & Mpagama, S. G. (2022). Use of a molecular bacterial load assay to distinguish between active TB and post-TB lung disease. International Journal of Tuberculosis and Lung Disease, 26(3), 276-278. https://doi.org/10.5588/ijtld.21.0459
- Mbelele, P. M., Mpolya, E. A., Sauli, E., Mtafya, B., Ntinginya, N. E., Addo, K. K., Kreppel, K., Mfinanga, S., Phillips, P. P. J., Gillespie, S. H., Heysell, S. K., Sabiiti, W., & Mpagama, S. G. (2021). Mycobactericidal effect of different regimens measured by molecular bacterial load assay among people treated for multidrug-resistant tuberculosis in Tanzania. Journal of Clinical Microbiology, 59(4), Article e02927-20. https://doi.org/10.1128/JCM.02927-20
- Said, B., Charlie, L., Getachew, E., Wanjiru, C.L., Abebe, M., Manyazewal, T. (2021) Molecular bacterial load assay versus culture for monitoring treatment response in adults with tuberculosis. SAGE Open Medicine. https://doi.org/10.1177/20503121211033470.
- Mbelele, P. M., Mpolya, E. A., Sauli, E., Mtafya, B., Ntinginya, N. E., Addo, K. K., Kreppel, K., Mfinanga, S., Phillips, P. P. J., Gillespie, S. H., Heysell, S. K., Sabiiti, W., & Mpagama, S. G. (2021). Mycobactericidal effect of different regimens measured by molecular bacterial load assay among people treated for multidrug-resistant tuberculosis in Tanzania. Journal of Clinical Microbiology, 59(4), Article e02927-20. https://doi.org/10.1128/JCM.02927-20
- Thanh Hai, H., Sabiiti, W., Anh Thu, D. D., Hoan Phu, N., Gillespie, S. H., Thwaites, G., & Thuy Thuong Thuong, N. (2021). Evaluation of the molecular bacterial load assay for detecting viable Mycobacterium tuberculosis in cerebrospinal fluid before and during tuberculous meningitis treatment. Tuberculosis, 128, Article 102084. https://doi.org/10.1016/j.tube.2021.102084
- Osei-Wusu, S., Morgan, P., Asare, P., Adams, G., Musah, A. B., Siam, I. M., Gillespie, S. H., Sabiiti, W., & Yeboah-Manu, D. (2021). Bacterial load comparison of the three main lineages of Mycobacterium tuberculosis complex in West Africa. Frontiers in Microbiology, 12, Article 719531. Advance online publication. https://doi.org/10.3389/fmicb.2021.719531
- Sabiiti, W., Azam, K., Farmer, E., Kuchaka, D., Mtafya, B., Bowness, R., Oravcova, K., Honeyborne, I., Evangelopoulos, D., McHugh, T. D., Khosa, C., Rachow, A., Heinrich, N., Kampira, E., Davies, G., Bhatt, N., Ntinginya, E. N., Viegas, S., Jani, I., … Gillespie, S. H. (2020). Tuberculosis bacillary load, an early marker of disease severity and treatment response: the utility of tuberculosis Molecular Bacterial Load Assay. Thorax, 75(7), 606-608. https://doi.org/10.1136/thoraxjnl-2019-214238
- Sabiiti, W., & MBLA development stakeholders (2019). United Kingdom–East and Southern Africa partnership at the forefront of developing first ever test that measures patient tuberculosis burden in hours. East Africa Science, 1(1), 3-8. https://doi.org/10.24248/EASci-D-18-00008
- Mtafya, B., Sabiiti, W., Sabi, I., John, J., Sichone, E., Ntinginya, N. E., & Gillespie, S. H. (2019). Molecular bacterial load assay (MBLA) concurs with culture on the NaOH-induced Mycobacterium tuberculosis loss of viability. Journal of Clinical Microbiology, 57, Article e01992-18. https://doi.org/10.1128/JCM.01992-18
- Svensson, R. J., Sabiiti, W., Kibiki, G. S., Ntinginya, N. E., Bhatt, N., Davies, G., Gillespie, S. H., & Simonsson, U. S. H. (2019). Model-based relationship between the molecular bacterial load assay and time-to-positivity in liquid culture. Antimicrobial Agents and Chemotherapy, 63(10), Article e00652-19. https://doi.org/10.1128/AAC.00652-19
- Sabiiti, W., Azam, K., Esmeraldo, E., Bhatt, N., Rachow, A., & Gillespie, S. H. (2019). Heat-inactivation renders sputum safe and preserves Mycobacterium tuberculosis RNA for downstream molecular tests. Journal of Clinical Microbiology, 57(4), Article e01778-18. https://doi.org/10.1128/JCM.01778-18
- Azam, K., Cadir, N., Madeira, C., Gillespie, S. H., & Sabiiti, W. (2018). OMNIgene.SPUTUM suppresses contaminants while maintaining Mycobacterium tuberculosis viability and obviates cold-chain transport. ERJ open research, 4(1), 00074-2017. https://doi.org/10.1183/23120541.00074-2017
- Gillespie, S. H., Sabiiti, W., & Oravcova, K. (2017). Mycobacterial load assay. In K. A. Bishop-Lilly (Ed.), Diagnostic Bacteriology: Methods and Protocols (pp. 89-105). (Methods in Molecular Biology; Vol. 1616). Humana Press/Springer. https://doi.org/10.1007/978-1-4939-7037-7_5
- Honeyborne, I., McHugh, T. D., Phillips, P., Bannoo, S., Bateson, A., Carroll, N., Perrin, F., Ronacher, K., Wright, L., van Helden, P., Walzl, G., & Gillespie, S. H. (2011). Molecular Bacterial Load Assay, a Culture-Free Biomarker for Rapid and Accurate Quantification of Sputum Mycobacterium tuberculosis Bacillary Load during Treatment. Journal of Clinical Microbiology, 49(11), 3905-3911. https://doi.org/10.1128/JCM.00547-11